JAK3 Protein (AA 781-1124)

Catalog No. ABIN7939218

This Recombinant JAK3 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for JAK3 Protein (AA 781-1124) (ABIN7939218)

Target: JAK3 (Janus Kinase 3 (JAK3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 781-1124

Purpose: Recombinant JAK3 (781-1124) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant JAK3 (781-1124) protein is supplied in 25 mM HEPESNaOH pH 7.5, 500 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: JAK3 (Janus Kinase 3 (JAK3))

Alternative Name: JAK3

Background:

Protein Background: JAK3 (Janus Kinase 3) a non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation and mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, JAK3 plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, JAK3 phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, JAK3 phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 Molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. Diseases associated with JAK3 include Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Negative and Nk-Cell Enteropathy

Short Description: Recombinant JAK3 (781-1124) protein that includes amino acids 781-1124 of human JAK3 protein (accession number NP_000206.2) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 39.8 kDa

Aliases: Janus Kinase 3

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response