c-MET Protein (AA 956-1390)

Catalog No. ABIN7939268

Recombinant c-MET protein expressed in Baculovirus infected Insect Cells.

Quick Overview for c-MET Protein (AA 956-1390) (ABIN7939268)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Product Details

Protein Characteristics: AA 956-1390

Purpose: Recombinant MET (956-1390) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MET (956-1390) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: MET

Background:

Protein Background: MET (Hepatocyte growth factor receptor), also known as AUTS9, c-Met, DA11, DFNB97, HGFR and RCCP2. MET is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinaseAKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts.

Short Description: Recombinant MET (956-1390) protein that includes amino acids 956-1390 of human MET protein (accession number NP_000236.2) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. The molecular weight of the protein is 50.30 kDa.

Aliases: Hepatocyte growth factor receptor, AUTS9, c-Met, DA11, DFNB97, HGFR, RCCP2

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor