MEK1 Protein

Catalog No. ABIN7939328

Recombinant MEK1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for MEK1 Protein (ABIN7939328)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Enzyme Kinetics (EK), Screening Assay (ScA)

Product Details

Purpose: Recombinant MAP2K1 (MEK1) protein

Application Details

Application Notes: Recombinant MAP2K1 (MEK1) protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MAP2K1 (MEK1) protein is supplied in 25 mM Tris-HCl pH 7.4, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background:

Protein Background: MAP2K1 (Mitogen-Activated Protein Kinase Kinase 1), also known as MEK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase, and is an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Short Description: Recombinant human MAP2K1 (MEK1) protein was expressed in baculovirus expression system as the full length protein (accession number NP_002746.1) with an N-terminal 6xHis tag. The molecular weight of the protein is 46.8 kDa.

Aliases: Mitogen-Activated Protein Kinase Kinase 1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling