MAP3K8 Protein (AA 30-397)

Catalog No. ABIN7939339

Recombinant MAP3K8 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for MAP3K8 Protein (AA 30-397) (ABIN7939339)

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 30-397

Purpose: Recombinant MAP3K8 (30-397) / COT protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MAP3K8 / COT (30-397) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8 & COT

Background:

Protein Background: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8) is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. It also involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells and involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro>, MAP3K8 activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner and leading to up-regulation of IL8 and CCL4 and transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages. Thus MAP3K8 may play a role in the regulation of immunoglobulin production and may also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, MAP3K8 activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF.

Short Description: Recombinant MAP3K8 / COT (30-397) protein that includes amino acids 30-397 of human MAP3K8 protein (accession number NP_001231063.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 42.8 kDa.

Aliases: (Mitogen-Activated Protein Kinase Kinase Kinase 8

Pathways: PI3K-Akt Signaling, TCR Signaling