MAP3K8 Protein (GST tag)
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- Target See all MAP3K8 Proteins
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This MAP3K8 protein is labelled with GST tag.
- Purpose
- Recombinant Human TPL2/MAP3K8/MEKK8 Protein (GST Tag)
- Sequence
- Met 30-Arg 397
- Characteristics
- A DNA sequence encoding the human MAP3K8 isoform 1 (P41279-1) (Met 30-Arg 397) was fused with the GST tag at the N-terminus.
- Purity
- > 91 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product MAP3K8 Protein
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Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 30-397) (Active) protein (GST tag)
MAP3K8 Origin: Human Host: Insect Cells Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 30-397) (Active) protein (His-GST)MAP3K8 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 1-467) protein (GST tag)MAP3K8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 368-467) protein (GST tag)MAP3K8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 1-467) protein (Strep Tag)Crystallography grade MAP3K8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 1-467) protein (Strep Tag)Crystallography grade MAP3K8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
- Alternative Name
- TPL2/MAP3K8/MEKK8 (MAP3K8 Products)
- Synonyms
- MAP3K8 Protein, COT Protein, EST Protein, ESTF Protein, MEKK8 Protein, TPL2 Protein, Tpl-2 Protein, c-COT Protein, Cot Protein, Cot/Tpl2 Protein, Est Protein, Estf Protein, Tpl2 Protein, D17TPL2 Protein, mitogen-activated protein kinase kinase kinase 8 Protein, MAP3K8 Protein, map3k8 Protein, Map3k8 Protein
- Background
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Background: Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).
Synonym: AURA2;c-COT;COT;EST;ESTF;MEKK8;Tpl-2;TPL2
- Molecular Weight
- 68 kDa
- Pathways
- PI3K-Akt Signaling, TCR Signaling
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