MGMT Protein
Quick Overview for MGMT Protein (ABIN7939403)
Target
See all MGMT ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant MGMT protein
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant MGMT protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 % glycerol and 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- MGMT
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Background
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Protein Background: MGMT(Methylated-DNA--protein-cysteine methyltransferase) Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Short Description: Recombinant MGMT protein that includes full length of human MGMT protein (accession number NP_002403.3) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 48.48 kDa.
Aliases: O-6-Methylguanine-DNA Methyltransferase
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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