PPARG Protein

Catalog No. ABIN7939414

This Recombinant PPARG protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for PPARG Protein (ABIN7939414)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant PPARg protein

Characteristics: PPARg (Peroxisome Proliferator Activated Receptor Gamma), also called as PPARG or NR1C3, is a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. PPAR-gamma is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, it can bind to DNA specific PPAR response elements (PPRE) and modulate the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. It's also a key regulator of adipocyte differentiation and glucose homeostasis. PPAR-gamma acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. It plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PPARg protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARg

Background:

Protein Background: PPARg (Peroxisome Proliferator Activated Receptor Gamma), also called as PPARG or NR1C3, is a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. PPARg is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, it can bind to DNA specific PPAR response elements (PPRE) and modulate the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. It's also a key regulator of adipocyte differentiation and glucose homeostasis. PPARg acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. It plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

Short Description: Recombinant PPARg protein was expressed in baculovirus expression system as the full length protein (accession number NP_056953.2) with an N-terminal FLAG tag. The molecular weight of the protein is 58.7 kDa.

Aliases: GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation