SUMO1 Protein

Catalog No. ABIN7939594

This Recombinant SUMO1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SUMO1 Protein (ABIN7939594)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant SUMO1 protein - biotinylated

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant SUMO1 protein - biotinylated is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1

Background:

Protein Background: SUMO1 (Small ubiquitin-related modifier 1) is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1, this modulates the gating characteristics of KCNB1.

Short Description: Recombinant SUMO1 protein - biotinylated that includes full length of human SUMO1 protein (accession number NP_001005781.1) was expressed in E. coli and contains an N-terminal His & Avi tag with a molecular weight of 15.81 kDa. This protein has been modified by biotin in vivo. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: Small ubiquitin-related modifier 1, SMT3H3, SMT3C, GMP1, SUMO-1, UBL1, DAP1, Sentin

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway