Vimentin Protein (VIM) (AA 1-466)

Catalog No. ABIN8057080

This Recombinant Vimentin protein is expressed in Mammalian Cells.

Quick Overview for Vimentin Protein (VIM) (AA 1-466) (ABIN8057080)

Target: Vimentin (VIM)

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: Immunogen (Imm)

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-466

Purpose: Recombinant Human VIM Protein(Halo Tag)

Sequence: Met1-Glu466

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Vimentin (VIM)

Alternative Name: VIM

Background: Vimentin,Vimentin is a type III intermediate filament(IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl(tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.

Molecular Weight:

Calculated MW: 93.1 kDa

Observed MW: 91 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis