CD73 Protein (AA 29-500)
Quick Overview for CD73 Protein (AA 29-500) (ABIN8126694)
Target
See all CD73 (NT5E) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 29-500
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Purpose
- Active Cluster Of Differentiation 73 (CD73)
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Sequence
- Leu29-Thr500
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) (Active) protein (His tag)
NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) (Active) protein (hFc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) (Active) protein (His tag)NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) (Active) protein (His tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-252) protein (His tag)NT5E Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 34-214) protein (His tag)NT5E Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-551) (Active) protein (His tag)NT5E Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, EAA Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 1-549) (Active) protein (His tag)NT5E Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 6.4
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Alternative Name
- CD73
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Background
- NT5E, NT5-E, E5NT, E5-NT, NTE, EN, ENT, 5'-Nucleotidase, Ecto
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Molecular Weight
- 53kDa
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UniProt
- P21589
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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