CD73 Protein (AA 29-551) (His tag)

Catalog No. ABIN5854793

Product Details

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 29-551

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CD73 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Nt5e (NT5E Products)

Synonyms: CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein

Background: Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of general functional importance for the metabolism of nucleotides at the ventricular surface of the retina as well as the ventricles of the brain, a feature that is maintained throughout development. Recombinant mouse Nt5e, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 59.1kDa (531aa) 50-70kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_035981

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process