Insulin Protein (INS) (AA 25-54)

Catalog No. ABIN8128107

This Recombinant Insulin protein is produced in Escherichia coli (E. coli).

Quick Overview for Insulin Protein (INS) (AA 25-54) (ABIN8128107)

Target: Insulin (INS)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cow

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC), Activity Assay (AcA)

Purity: > 90 %

Product Details

Protein Characteristics: AA 25-54

Purpose: Active Insulin (INS)

Sequence: Phe25-Ala54

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 6.4

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: Insulin (INS)

Alternative Name: Insulin

Molecular Weight: 36kDa

UniProt: P01317

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation