Sonic Hedgehog Protein (SHH) (AA 36-193)

Catalog No. ABIN8129300

Recombinant Sonic Hedgehog protein expressed in HEK-293F Cells.

Quick Overview for Sonic Hedgehog Protein (SHH) (AA 36-193) (ABIN8129300)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293F Cells

Application: Cell Culture (CC), Activity Assay (AcA)

Purity: > 97 %

Product Details

Protein Characteristics: AA 36-193

Purpose: Active Hedgehog Homolog, Sonic (SHH)

Sequence: Pro36-Ala193

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.1

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Hedgehog Homolog, Sonic

Background: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

Molecular Weight: 20kDa

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development