Quick Overview for PTPN1 Protein (ABIN934895)
Target
See all PTPN1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Sequence
- MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
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Characteristics
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Purified recombinant Human PTP1 B protein
Expression System: E.coli
Bioactivity: 10,000units/mg of PTP1B
Molecular weight on SDS-PAGE will appear higher.
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Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-435) protein (GST tag)
PTPN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (Active) protein (His tag)PTPN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-321) (Active) proteinPTPN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (DYKDDDDK Tag)PTPN1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (Myc-DYKDDDDK Tag)PTPN1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- PTP1B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Comment
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One unit will hydrolyze 1 nM of p-nitrophenyl-phosphatate per minute at pH 7.4 at 37 °C using 10 mM of substrate
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 2 mM beta-mercaptoethanol, 1 mM EDTA.1 mMDTT, and 20 % glycerol.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
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Alternative Name
- PTP1B
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Background
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The protein coding region of the catalytic domain of PTP-1B was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
Alternative Names: PTPN1 protein, PTPB 1 protein, Protein Tyrosine Phosphatase Non Receptor Type-1, PTPB-1, Protein Tyrosine Phosphatase 1B protein, PTP1B protein, PTPB 1, PTP-1B protein, EC 3.1.3.48 protein, PTP1B, PTPB-1 protein, Protein tyrosine phosphatase non-receptor type 1 protein, Protein-tyrosine phosphatase1B protein, Tyrosine-protein phosphatase non-receptor type 1 protein -
Molecular Weight
- 37.3 kDa (321 AA)
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Pathways
- TLR Signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling
Target
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