PTPN1 Protein (His tag)

Catalog No. ABIN7317110

Product Details

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PTPN1 protein is labelled with His tag.

Purpose: Recombinant Human PTP1B/PTPN1 Protein (His Tag)(Active)

Sequence: Glu 2-Asn 321

Characteristics: A DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2-Asn 321) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 15 nmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 10 mM Hepes, 150 mM NaCl, 1 mM DTT, pH 7.5

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Alternative Name: PTP1B/PTPN1 (PTPN1 Products)

Synonyms: PTPN1 Protein, PTP1B Protein, PTP-1B Protein, PTP-HA2 Protein, Ptp Protein, ptp1b Protein, wu:fk54h03 Protein, CPTP1 Protein, protein tyrosine phosphatase, non-receptor type 1 Protein, protein tyrosine phosphatase, non-receptor type 1 L homeolog Protein, PTPN1 Protein, Ptpn1 Protein, ptpn1 Protein, ptpn1.L Protein

Background:

Background: PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.

Synonym: PTP1B,PTPN1

Molecular Weight: 38 kDa

NCBI Accession: NP_002818

Pathways: TLR Signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling