Leptin Protein (LEP)
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- Target See all Leptin (LEP) Proteins
- Leptin (LEP)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
- Characteristics
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Purified recombinant Human Leptin protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Top Product
- Discover our top product LEP Protein
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- Application Notes
- Leptin protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in PBS, pH 7.4.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- Leptin (LEP)
- Alternative Name
- Leptin (LEP Products)
- Background
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Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: Obesity protein, OB protein, Obesity homolog mouse protein, Leptin protein, Leptin Precursor Obesity Factor protein, Obesity factor protein, Leptin protein, LEP protein, Obese protein protein, Obesity Murine Homolog Leptin protein, Leptin Murine Obesity Homolog protein, , OBS protein - Molecular Weight
- 16 kDa (147 AA)
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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