aHSP Protein (AA 1-102)
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- Target See all aHSP Proteins
- aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-102
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS
- Characteristics
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Purified recombinant Human AHSP protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) protein (GST tag)
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alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) protein (Strep Tag)Crystallography grade aHSP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
alpha Hemoglobin Stabilizing Protein (aHSP) proteinaHSP Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
alpha Hemoglobin Stabilizing Protein (aHSP) protein (Myc-DYKDDDDK Tag)aHSP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- AHSP protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))
- Alternative Name
- AHSP (aHSP Products)
- Synonyms
- AHSP Protein, ERAF Protein, EDRF Protein, Eraf Protein, alpha hemoglobin stabilizing protein Protein, alpha-hemoglobin-stabilizing protein-like Protein, AHSP Protein, Ahsp Protein, LOC100620111 Protein
- Background
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AHSP(Alpha-hemoglobin stabilizing protein), also known as ERAF(Erythroid associated factor), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of alpha-hemoglobin during normal erythroid cell development. It specifically protects free alpha-hemoglobin from precipitation in live cells and in solution. This protein is downregulated in transmissible spongiform encephalopathies (TSEs). It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Recombinant AHSP protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Erythroid associated factor protein, , Erythroid differentiation associated factor protein, Erythroid differentiation related factor protein, Alpha-hemoglobin stabilizing protein Alpha hemoglobin stabilizing protein protein, ERAF protein, EDRF protein - Molecular Weight
- 11.8 kDa (102 AA)
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