PTS Protein (AA 1-145) (His tag)
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- Target See all PTS Proteins
- PTS (6-Pyruvoyltetrahydropterin Synthase (PTS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-145
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PTS protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSTEGGGRRC QAQVSRRISF SASHRLYSKF LSDEENLKLF GKCNNPNGHG HNYKVVVTVH GEIDPATGMV MNLADLKKYM EEAIMQPLDH KNLDMDVPYF ADVVSTTENV AVYIWDNLQK VLPVGVLYKV KVYETDNNIV VYKGE
- Characteristics
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Purified recombinant Human PTS protein
Expression System: E.coli - Purity
- > 90 % pure
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6-Pyruvoyltetrahydropterin Synthase (PTS) (AA 1-145) protein (GST tag)
PTS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
6-Pyruvoyltetrahydropterin Synthase (PTS) (AA 1-100) protein (GST tag)PTS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
6-Pyruvoyltetrahydropterin Synthase (PTS) (AA 1-144) protein (Strep Tag)Crystallography grade PTS Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
6-Pyruvoyltetrahydropterin Synthase (PTS) protein (Myc-DYKDDDDK Tag)PTS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- PTS protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 20 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- PTS (6-Pyruvoyltetrahydropterin Synthase (PTS))
- Alternative Name
- PTS (PTS Products)
- Synonyms
- PTS Protein, Pts Protein, DDBDRAFT_0232952 Protein, DDB_0232952 Protein, ptps Protein, zgc:162276 Protein, PTPS Protein, 6-pyruvoyltetrahydropterin synthase Protein, 6-pyruvoyltetrahydropterin synthase L homeolog Protein, 6-pyruvoyl-tetrahydropterin synthase Protein, PTS Protein, pts.L Protein, Pts Protein, azo3118 Protein, Xfasm12_0164 Protein, HUTA_RS09790 Protein, Cyan8802_3683 Protein, ptsA Protein, NMAG_RS16995 Protein, pts Protein, DICPUDRAFT_151723 Protein
- Background
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6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: FLJ97081 protein, 6-pyruvoyltetrahydropterin synthase 6 pyruvoyl tetrahydrobiopterin synthase protein, EC 4.2.3.12 protein, PTP synthase protein, 6 pyruvoyl tetrahydropterin synthase protein, , 6 pyruvoyltetrahydropterin synthase protein, PTPS protein - Molecular Weight
- 18.5 kDa (165 AA)
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