TNF alpha Protein (full length)
(1 reference)Quick Overview for TNF alpha Protein (full length) (ABIN935842)
Target
See all TNF alpha ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- full length
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Characteristics
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Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is -
Purification
- Proprietary chromatographic technique
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)custom-made TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)custom-made TNF alpha Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Assay Procedure
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1 - 1.0 g/mL.
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Buffer
- Lyophilized from 20 mM PBS, pH 7.2, with 10 mM NaCl.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).
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: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- TNF alpha
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Background
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Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Alternative Names: Tumor Necrosis Factor protein, TNFa protein, Cytotoxin protein, DIF protein, TNFSF2 protein, TNF-alpha protein, TNF-a protein, Differentiation-inducing factor protein, Necrosin protein, Cachectin protein -
Molecular Weight
- 17,483 Da
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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