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Hirudin Protein

Origin: Leech Host: Yeast (Pichia pastoris) Recombinant > 96 % by SDS-PAGE and HPLC analyses. Active
Catalog No. ABIN987947
  • Target See all Hirudin products
    Hirudin
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    Leech
    Source
    Yeast (Pichia pastoris)
    Sequence
    VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EY
    Characteristics
    The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 10,000ATU/mg protein.
    Purity
    > 96 % by SDS-PAGE and HPLC analyses.
    Endotoxin Level
    Level Less than 10EU/mg of rhirudin as determined by LAL method
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Storage
    4 °C
  • Target
    Hirudin
    Abstract
    Hirudin Products
    Background
    Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. Synonym: Hirudin. Formulation: Lyophilized from a 0.2µm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
    Molecular Weight
    Approximately 6.7k Da, containing 63 amino acid residues.
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