|Antigen||Netrin 1 (NTN1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Netrin 1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-Netrin 1 AntibodyTarget Details Netrin 1 Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) Images
|Purification||Immunogen affinity purified|
|Immunogen||Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.|
|Plasmids, Primers & others|
Target Details Netrin 1Product Details anti-Netrin 1 Antibody Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) Images back to top
|Alternative Name||Netrin-1 (NTN1 Antibody Abstract)|
|Background||Gene Symbol: NTN1|
|Pathways||Regulation of Cell Size|
Application DetailsProduct Details anti-Netrin 1 Antibody Target Details Netrin 1 Handling References for anti-Netrin 1 antibody (ABIN151152) Images back to top
|Application Notes||Western Blot, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:1000-1:2000, Immunohistochemistry-Paraffin 1:1000-1:2000, Immunohistochemistry-FrozenUse in Immunohistochemistry-Frozen reported in scientific literature (PMID 26482371).|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details References for anti-Netrin 1 antibody (ABIN151152) Images back to top
10 mM Sodium PBS (0.9 % isotonic, w/v, pH 7.2)
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 4°C in the dark.|
References for anti-Netrin 1 antibody (ABIN151152)Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Handling Images back to top
|Product cited in:||
Salameh, Nouel, Flores, Hoops: "An optimized immunohistochemistry protocol for detecting the guidance cue Netrin-1 in neural tissue." in: MethodsX, Vol. 5, pp. 1-7, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).
Reynolds, Pokinko, Torres-Berrío, Cuesta, Lambert, Del Cid Pellitero, Wodzinski, Manitt, Krimpenfort, Kolb, Flores: "DCC Receptors Drive Prefrontal Cortex Maturation by Determining Dopamine Axon Targeting in Adolescence." in: Biological psychiatry, Vol. 83, Issue 2, pp. 181-192, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).
Wu, Yuan, Gao, Li, Cao, Liu, Yu, Tian, Meng, Shi, Pan, Li: "Netrin-1 Contributes to Myelinated Afferent Fiber Sprouting and Neuropathic Pain." in: Molecular neurobiology, Vol. 53, Issue 8, pp. 5640-51, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)
Manitt, Mimee, Eng, Pokinko, Stroh, Cooper, Kolb, Flores: "The netrin receptor DCC is required in the pubertal organization of mesocortical dopamine circuitry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 23, pp. 8381-94, 2011
Manitt, Labelle-Dumais, Eng, Grant, Mimee, Stroh, Flores: "Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents." in: PLoS ONE, Vol. 5, Issue 7, pp. e11463, 2010 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence,Immunohistochemistry
Tallman, Kim, Montesinos, Appelbaum, de la Serna, Bennett, Deben, Bloomfield, Gonzalez, Feusner, Gonzalez, Gallagher, Miguel, Larson, Milone, Paietta, Rayon, Rowe, Rivas, Schiffer, Vellenga, Shepherd et al.: "Does microgranular variant morphology of acute promyelocytic leukemia independently predict a less favorable outcome compared with classical M3 APL? A joint study of the North American Intergroup and ..." in: Blood, Vol. 116, Issue 25, pp. 5650-9, 2010 (Sample species: Human).
ImagesProduct Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) back to top