anti-Rat (Rattus) Netrin 1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Netrin 1 Antibody

Antigen
Netrin 1 (NTN1) Antibodies
  • ntn1-A
  • Netrin-1
  • NTN1
  • unc-6
  • MGC147073
  • NTN1L
  • AI561871
  • BC019633
  • netrin-1
  • netrin 1 L homeolog
  • netrin 1
  • ntn1.L
  • NTN1
  • ntn1
  • Ntn1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
94
80
62
10
10
6
4
4
3
2
2
1
1
Host
Chicken
79
14
11
5
4
2
1
Clonality
Polyclonal
Conjugate
This Netrin 1 antibody is un-conjugated
6
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
76
40
35
16
13
9
7
7
4
3
3
2
Options

Available images

Catalog No. ABIN151152
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General

Antigen Netrin 1 (NTN1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(94), (80), (62), (10), (10), (6), (4), (4), (3), (2), (2), (1), (1)
Host Chicken
(79), (14), (11), (5), (4), (2), (1)
Clonality
Polyclonal
Conjugate This Netrin 1 antibody is un-conjugated
(6), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(76), (40), (35), (16), (13), (9), (7), (7), (4), (3), (3), (2)
Pubmed 6 references available
Supplier

Product Details anti-Netrin 1 Antibody

Target Details Netrin 1 Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) Images
Specificity Netrin-1
Purification Immunogen affinity purified
Immunogen Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.
Plasmids, Primers & others

Target Details Netrin 1

Product Details anti-Netrin 1 Antibody Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) Images back to top
Antigen
Alternative Name Netrin-1 (NTN1 Antibody Abstract)
Background Gene Symbol: NTN1
Gene ID 9423
UniProt O95631, O09118
Pathways Regulation of Cell Size

Application Details

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Handling References for anti-Netrin 1 antibody (ABIN151152) Images back to top
Application Notes Western Blot, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:1000-1:2000, Immunohistochemistry-Paraffin 1:1000-1:2000, Immunohistochemistry-FrozenUse in Immunohistochemistry-Frozen reported in scientific literature (PMID 26482371).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details References for anti-Netrin 1 antibody (ABIN151152) Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer 10 mM Sodium PBS (0.9 % isotonic, w/v, pH 7.2)
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.

References for anti-Netrin 1 antibody (ABIN151152)

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Handling Images back to top
Product cited in:

Salameh, Nouel, Flores, Hoops: "An optimized immunohistochemistry protocol for detecting the guidance cue Netrin-1 in neural tissue." in: MethodsX, Vol. 5, pp. 1-7, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Reynolds, Pokinko, Torres-Berrío, Cuesta, Lambert, Del Cid Pellitero, Wodzinski, Manitt, Krimpenfort, Kolb, Flores: "DCC Receptors Drive Prefrontal Cortex Maturation by Determining Dopamine Axon Targeting in Adolescence." in: Biological psychiatry, Vol. 83, Issue 2, pp. 181-192, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Wu, Yuan, Gao, Li, Cao, Liu, Yu, Tian, Meng, Shi, Pan, Li: "Netrin-1 Contributes to Myelinated Afferent Fiber Sprouting and Neuropathic Pain." in: Molecular neurobiology, Vol. 53, Issue 8, pp. 5640-51, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Manitt, Mimee, Eng, Pokinko, Stroh, Cooper, Kolb, Flores: "The netrin receptor DCC is required in the pubertal organization of mesocortical dopamine circuitry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 23, pp. 8381-94, 2011

Manitt, Labelle-Dumais, Eng, Grant, Mimee, Stroh, Flores: "Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents." in: PLoS ONE, Vol. 5, Issue 7, pp. e11463, 2010 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence,Immunohistochemistry

Tallman, Kim, Montesinos, Appelbaum, de la Serna, Bennett, Deben, Bloomfield, Gonzalez, Feusner, Gonzalez, Gallagher, Miguel, Larson, Milone, Paietta, Rayon, Rowe, Rivas, Schiffer, Vellenga, Shepherd et al.: "Does microgranular variant morphology of acute promyelocytic leukemia independently predict a less favorable outcome compared with classical M3 APL? A joint study of the North American Intergroup and ..." in: Blood, Vol. 116, Issue 25, pp. 5650-9, 2010 (Sample species: Human).

Images

Product Details anti-Netrin 1 Antibody Target Details Netrin 1 Application Details Handling References for anti-Netrin 1 antibody (ABIN151152) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Netrin 1 (NTN1) antibody (ABIN151152) Dissociated cell cultures of an e 13 mouse brain showing Netrin-1 (green staining) in...