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Rockland Antibodies

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TUBA1B antibody (C-Term)

Rockland 200-301-880 TUBA1B Reactivity: Human WB, ELISA, IF, IHC, FM, MA Host: Mouse Monoclonal 17H11-F10 unconjugated

Scx is required for the expression of Fmod and Adamtsl2.

Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibody (ABIN964563)

Immunofluorescence of Mouse monoclonal anti-Alpha Tubulin antibody.

Immunofluorescences of Mouse monoclonal anti-Alpha Tubulin antibody.

HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 µg of lysate each.

Smad3 is required for TGF-β to regulate expression of Fmod and Adamtsl2.

TGF-β signaling regulates noncanonical pathways in the sclerotome.

AKT and p38 are not required for TGF-β-mediated regulation of fibrous tissue markers.

ERK is required for fibrous tissue marker regulation but not required to inhibit chondrogenesis.

13 images

6 references

Catalog No. ABIN964563

$389.00

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100 μg

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Rockland Original

His Tag antibody

Rockland 600-401-382 WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated

A knock-in mouse line with NALCN tagged with GFP, HA, and His tags (NALCN-GFP-HA-His mice).

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody.

Crude extracts prepared from Salmonella strains with the phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies.

$Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads.$ WB

5 images

14 references

Catalog No. ABIN100492

$345.00

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100 μg (More quantities available)

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Rockland Original

NF-kB p65 antibody (C-Term)

Rockland 200-301-065 NFkBP65 Reactivity: Human WB, IHC, ELISA, IF, FM Host: Mouse Monoclonal 27F9-G4 unconjugated

anti NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate.

Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues.

IHC

Anti NFkB monoclonal antibody – Immunocytochemistry Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: 600-401-271 anti p65 NLS specific lot 18372 Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein.

Immunofluorescence (IF) image for anti-Nuclear Factor-kB p65 (NFkBP65) (C-Term) antibody (ABIN964680)

Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 in b.

monoclonal anti p65 was used to detect p65 by Western blot.

6 images

2 references

Catalog No. ABIN964680

$522.00

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100 μg (More quantities available)

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Rockland Original

PDCD4 antibody (pSer457)

Rockland 600-401-964 PDCD4 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western blot using affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads).

affinity purified anti-Pdcd4 pS457 antibody was used at 1.

IHC

Immunohistochemical analysis of PDCD4 shows the corresponding H&E-stained and PDCD4-stained tissue sections from patients with OSCC.

IHC

PDCD4 mRNA and PDCD4 protein levels in OSCC cell lines.

Western blotting analysis demonstrating over-expression or knock-down of PDCD4 using PDCD4 plasmid or PDCD4 targeted siRNA, respectively, versus control plasmids (PCMV6, siRNA ctrl) in the UT-SCC cell lines (A) 24A, (B) 74A, and (C) 87.

IHC

Panel A shows miR-21 expression in pre-miR-21 or anti-miR-21 transfected cells compared to control (miR-scramble) in the UT-SCC cell lines 24A, 74A, and 87.

The upper panel shows miR-21 expression levels following transfection with pre-miR21, PDCD4 and PDCD4-UTRmut, compared to controls: scramble miR and PCMV6 empty vector.

14 images

3 references

Catalog No. ABIN129708

$509.00

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100 μg (More quantities available)

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Rockland Original

AHSA1 antibody (Internal Region)

Rockland 600-401-974 AHSA1 Reactivity: Human, Monkey WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Assembly of complexes of Cdc37 and Hsp90 phosphomimetic variants with clients and cochaperones.

Western blot using affinity purified anti-AHA1 antibody shows detection of AHA1 in Cos7 cells.

Western Blot of Rabbit anti-AHA1 antibody.

Interaction of KEAP1 and RHOBTB2 with HSP90 isoforms.

High levels of FNIPs make renal tumours sensitive to Hsp90 inhibitor GB.

8 images

4 references

Catalog No. ABIN233816

$509.00

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100 μg

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Rockland Original

HDAC1 antibody (AA 450-482)

Rockland 600-401-879 HDAC1 Reactivity: Human WB, IHC, ELISA, IF, FM, MA Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-HDAC-1 Antibody.

Immunofluorescence Microscopy of Rabbit anti-HDAC1 Antibody.

Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody.

IHC

6 images

3 references

Catalog No. ABIN129652

$509.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

100 μg (More quantities available)

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Rockland Original

TUBA1B antibody (AA 425-451)

Rockland 600-401-880 TUBA1B Reactivity: Human, Mouse, Rat WB, ELISA, IF, IHC, FM Host: Rabbit Polyclonal unconjugated

miR-155 and FOXP3 down regulate endogenous ZEB2 in human breast cancer cells resulting in altered levels of EMT markers Vimentin and E-cadherin(A) Relative abundance of ZEB2 and ZEB1 protein in WT, GFP or FOXP3 overexpressing BT549 cells transfected with miR-155 or miR-control.

Immunofluorescence microscopy of Rabbit Anti-alpha-Tubulin antibody using HeLa cells fixed with PFA.

ELISA

Western Blot of Rabbit anti-alpha-Tubulin antibody.

5 images

18 references

Catalog No. ABIN129653

$410.00

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200 μg (More quantities available)

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Rockland Original

TJP1 antibody

Rockland 600-401-gu7 TJP1 Reactivity: Human WB, IHC, ELISA, IF, FACS, FM, MA Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit anti-ZO-1 antibody Western Blot of Rabbit Anti-ZO-1 Antibody.

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody.

Immunohistochemistry of Rabbit anti-ZO-1 antibody Immunohistochemistry of Rabbit anti-ZO-1 antibody.

IHC

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody.

Immunofluorescence microscopy of ZO-1 Immunofluorescence microscopy of Anti-ZO-1 in Caco-2 cells using FITC-conjugated Fluorescent anti-rabbit IgG for detection.

6 images

1 reference

Catalog No. ABIN6655949

$509.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

100 μg

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Rockland Original

F11R antibody (pTyr280)

Rockland 600-401-gn5 F11R Reactivity: Human WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-JAM-A pY280 antibody with PP2.

Immunofluorescence Microscopy of Rabbit anti-JAMA pY280 antibody.

Western Blot of Rabbit anti-JAM A pY280 antibody.

Confocal Immunofluorescence Microscopy of Rabbit Anti-JAM-A pY280 antibody of confluent (intestinal epithelial cells) IECs.

Confocal Immunofluorescence Microscopy of Rabbit Anti-JAM-A pY280 antibody in polarized epithelial cells.

Western Blot of Rabbit Anti-JAM-A pY280 antibody with IL-22.

7 images

2 references

Catalog No. ABIN5706868

$509.00

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100 μg

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Rockland Original

Histone 3 antibody (H3K9ac)

Rockland 600-401-i72 H3 Reactivity: Human, Mouse, C. elegans WB, IHC, IF, ChIP, DB, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody.

Immunofluorescence of Rabbit Anti-Histone H3 [ac Lys9] Antibody.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys9] Antibody.

ChIP

Dot Blot of Rabbit Histone H3 [ac Lys9] Antibody.

6 images

1 reference

Catalog No. ABIN5706781

$532.00

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50 μg

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Rockland Original

Acetylated Lysine antibody

Rockland 600-401-939 Reactivity: Various Species WB, ELISA, IHC, IP, FACS, FM Host: Rabbit Polyclonal unconjugated

RAD54 acetylation is important for BRD9 recognition and HR activity.

Affinity Purified anti-Acetylated Lysine (AcK) antibody is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).

SIRT1 deacetylates KAP1 in vitro and in vivo.

Acetylation does not crosstalk with IR-induced pSer824 and does not affect the co-repressor activity of KAP1.

9 images

3 references

Catalog No. ABIN5596809

$509.00

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100 μg

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Rockland Original

NOTCH2 antibody

Rockland 100-401-408 NOTCH2 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue.

IHC

2 images

3 references

1 validation

Catalog No. ABIN104899

$509.00

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200 μL (More quantities available)

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Independently Validated

SIX3 antibody (Internal Region)

Rockland 200-201-a26s SIX3 Reactivity: Mouse WB, ELISA, IHC, IF, FM, DB Host: Guinea Pig Polyclonal unconjugated

TCF7L2 controls the establishment of anatomical borders in the thalamus and habenula.

IF (p)

Anti-Six3 Antibody - Immunofluorescence Microscopy Immunofluorescence microscopy using anti-Six3 antibody shows detection of Six3 in PFA-fixed embryonic mouse hypothalamus.

Immunofluorescence microscopy using Protein A purified anti-Six3 antibody shows detection of Six3 in various E14.

5 images

5 references

Catalog No. ABIN6657911

$125.00

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25 μL

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Rockland Original

GLI1 antibody (AA 805-820)

Rockland 100-401-223 GLI1 Reactivity: Mouse WB, ELISA, IHC, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blot - Gli1 Antibody Western blot using anti-Gli-1 antibody shows detection of a band at ~150 kDa (arrowhead) corresponding to human Gli-1 present in transfected 293T cell lysates (lanes 2 and 4).

Anti-Gli-1 Antibody - Immunofluorescence.

Inhibition of BRAFV600E, expression of GLI1 and SHH-GLI pathway inhibition by NVP-LDE225 in human melanoma cells in vitro.

4 images

12 references

Catalog No. ABIN6657594

$509.00

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100 μL

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Rockland Original

AKT1 antibody (C-Term)

Rockland 100-401-401 AKT1 Reactivity: Human, Mouse, Rat, Chicken WB, IHC, ELISA, IF, FACS, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-AKT antibody.

Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody.

Immunohistochemistry of Rabbit Anti-AKT antibody.

IHC

5 images

4 references

Catalog No. ABIN94783

$509.00

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200 μL (More quantities available)

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Rockland Original

COL1A1 antibody (Biotin)

Rockland 600-406-103 COL1A1 Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IP, FACS, DB, MA Host: Rabbit Polyclonal Biotin

Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody.

5 images

4 references

Catalog No. ABIN5596821

$532.00

Plus shipping costs $50.00

100 μg

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Rockland Original

PARP1 antibody (N-Term)

Rockland 200-401-gm8 PARP1 Reactivity: Human WB, IHC, 2D-PAGE Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit anti-PARP1-ZF with protein Western Blot of recombinant PARP1 with rabbit anti-PARP1 (N-term ZF1) antibody.

Western Blot of Rabbit anti-PARP1 antibody multi lysate Western Blot of Rabbit anti-PARP1 N-term Antibody.

Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies.

WB of Rabbit anti-PARP1 multi lysate Western Blot of Rabbit anti-PARP1 antibody.

2D SDS-PAGE and WB of PARP1 OVCAR-8 Wild Type Lysate was separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the zinc finger 1 domain of PARP1.

SDS

Western Blot of Rabbit anti-PARP1-ZF with lysate Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 (N-term ZF1) Antibody.

6 images

Catalog No. ABIN5706805

$509.00

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100 μg

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Rockland Original

PARP1 antibody (C-Term)

Rockland 200-401-x51 PARP1 Reactivity: Human WB, 2D-PAGE Host: Rabbit Polyclonal unconjugated

Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody.

Western Blot of Rabbit anti-PARP1 multi lysate Western Blot of Rabbit anti-PARP1 antibody.

2D SDS-PAGE and WB of PARP1 OVCAR-8 Wild Type Lysate separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the autocatalytic domain of PARP1.

SDS

Western Blot of Rabbit Anti-PARP1 (internal) Antibody.

Peggy Size Separation Electropherogram of Anti-PARP1 (internal).

6 images

Catalog No. ABIN5596914

$509.00

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100 μg

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Rockland Original

HA-Tag antibody

Rockland 600-401-384 WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue.

IHC

3 images

35 references

Catalog No. ABIN100176

$345.00

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100 μg (More quantities available)

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Rockland Original

MYL12A antibody (N-Term)

Rockland 600-401-416 MYL12A Reactivity: Human, Mouse WB, IHC, ELISA, IP Host: Rabbit Polyclonal unconjugated

Western blot of Rabbit Anti-Myosin pS19/pS20 primary antibody.

affinity purified anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.

IHC

Immunohistochemistry with anti-myosin pS19/pS20 antibody showing strong cytoplasmic staining of myocytes in mouse heart muscle 20x and 40x (B & C).

IHC

Affinity purified phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates.

Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot.

5 images

9 references

Catalog No. ABIN104491

$509.00

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100 μg (More quantities available)

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Rockland Original

Nestin antibody (AA 1484-1500)

Rockland 600-401-417 NES Reactivity: Human, Mouse IHC, WB, ELISA Host: Rabbit Polyclonal unconjugated

Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead).

Immunohistochemistry with anti-nestin antibody showing nestin staining in cytoplasm of of ductal epithelium of human salivary gland (B) and in nucleus and cytoplasm of human breast tissue (C).

IHC

ELISA

Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to human Nestin (arrowhead).

Immunohistochemistry with Anti-nestin antibody Tissue: mouse kidney (Left) and human kidney (Right).

IHC

5 images

5 references

Catalog No. ABIN104564

$509.00

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100 μg (More quantities available)

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Rockland Original

AKT3 antibody (Internal Region) (FITC)

Rockland 200-302-e75 AKT3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, DB, FACS, FM Host: Mouse Monoclonal 25F6-F6-D8 FITC

Western Blot of Mouse anti-AKT3 antibody.

Dot Blot of Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated.

Immunohistochemistry of Mouse Anti-AKT3 antibody.

IHC

Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney.

IHC

6 images

Catalog No. ABIN5596898

$532.00

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50 μg

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Rockland Original

Aurora Kinase B antibody (Internal Region)

Rockland 600-401-677 AURKB Reactivity: Human, Monkey IHC, WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size).

Bufalin prevents Aurora A recruitment to mitotic centrosomes and Aurora B recruitment to unattached kinetochores(A) HeLa cells were synchronized by a single thymidine treatment, released in the presence or absence of bufalin (100 nM) for 9 h, and stained for phospho-Aurora A (Green), α-tubulin (Red) and DNA (Blue).

IF (cc)

Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody.

IHC

High Aurora-B activity promotes KT attachment to MT-walls.

IF (cc)

4 images

18 references

Catalog No. ABIN129612

$509.00

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100 μg (More quantities available)

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Rockland Original

ROBO1 antibody (AA 1625-1650)

Rockland 600-401-692 ROBO1 Reactivity: Human, Mouse WB, ELISA, IHC, IP, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit anti-Robo-1 antibody.

Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.

IHC

Western blot using Affinity Purified anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead).

ELISA

5 images

1 reference

Catalog No. ABIN129621

$509.00

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100 μg (More quantities available)

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Rockland Original

AKT3 antibody (Internal Region)

Rockland 200-301-e75 AKT3 Reactivity: Human, Mouse WB, ELISA, IHC, FACS Host: Mouse Monoclonal 25F6-F6-D8 unconjugated

IHC

Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney.

IHC

5 images

1 reference

Catalog No. ABIN1043749

$522.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

100 μg (More quantities available)

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Rockland Original

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