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|Antigen||EPH Receptor B3 (EPHB3) Antibodies|
|Epitope||AA 1-537 Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This EPH Receptor B3 antibody is un-conjugated Alternatives|
|1 reference available|
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Product Details anti-EPH Receptor B3 AntibodyTarget Details EPH Receptor B3 Application Details Handling References for anti-EPH Receptor B3 antibody (ABIN2001175) Images
|Specificity||Mouse EphB3 / HEK2|
|Characteristics||Produced in rabbits immunized with purified, recombinant Mouse EphB3 / HEK2 (rM EphB3 / HEK2, ABIN2008000 NP_034273.1, Met 1-Thr 537,. EphB3 / HEK2 specific IgG was purified by Mouse EphB3 / HEK2 affinity chromatography.|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Mouse EphB3 / HEK2 protein (ABIN2008000)|
Target Details EPH Receptor B3Product Details anti-EPH Receptor B3 Antibody Application Details Handling References for anti-EPH Receptor B3 antibody (ABIN2001175) Images back to top
|Alternative Name||EPHB3 (EPHB3 Antibody Abstract)|
|Background||Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.|
Application DetailsProduct Details anti-EPH Receptor B3 Antibody Target Details EPH Receptor B3 Handling References for anti-EPH Receptor B3 antibody (ABIN2001175) Images back to top
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse EPHB3. The detection limit for Mouse EPHB3 is approximately 0.00975 ng/well.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor B3 Antibody Target Details EPH Receptor B3 Application Details References for anti-EPH Receptor B3 antibody (ABIN2001175) Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
References for anti-EPH Receptor B3 antibody (ABIN2001175)Product Details anti-EPH Receptor B3 Antibody Target Details EPH Receptor B3 Application Details Handling Images back to top
Bergemann, Zhang, Chiang, Brambilla, Klein, Flanagan: "Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube." in: Oncogene, Vol. 16, Issue 4, pp. 471-80, 1998