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|Antigen||KIT Ligand (KITLG) Antibodies|
|Reactivity||Rat (Rattus), Mouse (Murine), Human Alternatives|
|Conjugate||This KIT Ligand antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Western Blotting (WB)
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Product Details anti-KIT Ligand AntibodyTarget Details KIT Ligand Application Details Handling Images
|Specificity||SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate|
|Cross-Reactivity (Details)||SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate|
|Immunogen||KLH-coupled synthetic peptide from N-terminal of human SCF|
Target Details KIT LigandProduct Details anti-KIT Ligand Antibody Application Details Handling Images back to top
|Alternative Name||SCF (KITLG Antibody Abstract)|
Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis, and fertility. It exerts its activity at the early stages of hematopoiesis. Specifically, SCF stimulates the proliferation of mast cell and mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. In addition, SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways.
SCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583).
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user optimized
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
|Buffer||PBS, pH 7.4, containing 0.02 % sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Storage||4 °C/-20 °C|
|Storage Comment||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
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