anti-Mouse (Murine) Ret Proto-Oncogene antibody for Flow Cytometry

Recommended Ret Proto-Oncogene Antibody (supplied by: Log in to see )

Antigen
Ret Proto-Oncogene (RET) Antibodies
  • c-ret
  • cret
  • etID315074.13
  • ret1
  • wu:fd13h01
  • X-ret
  • ret-A
  • xret
  • RET
  • MTC1
  • CDHF12
  • CDHR16
  • HSCR1
  • MEN2A
  • MEN2B
  • PTC
  • RET-ELE1
  • RET51
  • RET9
  • c-Ret
  • ret proto-oncogene receptor tyrosine kinase
  • ret proto-oncogene
  • ret
  • RET
  • Ret
Epitope
AA 29-635
53
41
29
28
25
22
22
15
14
14
12
11
8
8
8
8
8
8
6
5
4
4
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
395
117
106
9
3
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
246
147
5
2
1
1
Clonality
Monoclonal
Conjugate
This Ret Proto-Oncogene antibody is un-conjugated
17
15
15
12
12
9
9
9
8
8
8
5
5
5
5
5
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
273
143
93
83
65
60
27
8
6
5
4
4
3
3
1
1
Supplier
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Catalog No. ABIN325734
$ 1,864.50
Plus shipping costs $45.00

General

Antigen Ret Proto-Oncogene (RET) Antibodies
Epitope AA 29-635
(53), (41), (29), (28), (25), (22), (22), (15), (14), (14), (12), (11), (8), (8), (8), (8), (8), (8), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(395), (117), (106), (9), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(246), (147), (5), (2), (1), (1)
Clonality
Monoclonal
Conjugate This Ret Proto-Oncogene antibody is un-conjugated
(17), (15), (15), (12), (12), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(273), (143), (93), (83), (65), (60), (27), (8), (6), (5), (4), (4), (3), (3), (1), (1)
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Product Details anti-Ret Proto-Oncogene Antibody

Target Details Ret Proto-Oncogene Application Details Handling Images
Specificity Recognizes human Ret. Species cross-reactivity: Mouse.
Purification Protein G purified
Immunogen Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).

Type of Immunogen: Recombinant protein
Isotype IgG1

Target Details Ret Proto-Oncogene

Product Details anti-Ret Proto-Oncogene Antibody Application Details Handling Images back to top
Antigen
Alternative Name RET (RET Antibody Abstract)
Background Name/Gene ID: RET
Subfamily: Cadherin
Family: Protein Kinase

Synonyms: RET, Cadherin family member 12, CDHF12, C-ret, Hirschsprung disease 1, HSCR1, Hydroxyaryl-protein kinase, Oncogene ret, Proto-oncogene c-Ret, RET-ELE1, RET51, Ret proto-oncogene, RET transforming sequence, RET43, Men2-ret, MEN2B, MTC1, Receptor tyrosine kinase, CDHR16, MEN2A, PTC, RET9
Gene ID 5979
UniProt P07949
Research Area Signaling, Kinases/Phosphatases
Pathways RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-Ret Proto-Oncogene Antibody Target Details Ret Proto-Oncogene Handling Images back to top
Application Notes Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 μg/mL, For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Western Blot: 1-2 μg/mL, Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhRet and rmRet is ~6 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-Ret Proto-Oncogene Antibody Target Details Ret Proto-Oncogene Application Details Images back to top
Format Lyophilized
Reconstitution Sterile PBS
Concentration Lot specific
Buffer Lyophilized from PBS, 5 % trehalose.
Handling Advice Avoid freeze/thaw cycles.
Storage -20 °C
Storage Comment Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Images

Product Details anti-Ret Proto-Oncogene Antibody Target Details Ret Proto-Oncogene Application Details Handling back to top
Supplier Images
 image for anti-Ret Proto-Oncogene (RET) (AA 29-635) antibody (ABIN325734) anti-Ret Proto-Oncogene (RET) (AA 29-635) antibody