Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN102221

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Cross-Reactivity: Sheep (Ovine)

Characteristics: Anti-Sheep IgG (H&L) antibody generated in donkey detects specifically sheep IgG heavy and light chains (H&L). This Anti sheep antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays. Anti-Sheep IgG (H&L) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Antibody Sheep IgG (H&L) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

ELISA Dilution: 1:2,000 - 1:8,000

Western Blot Dilution: 1:1,000 - 1:4,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Donkey Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody Alk phos Conjugation

Background: Anti-Sheep IgG (H&L) antibody generated in donkey detects specifically sheep IgG heavy and light chains (H&L). This Anti sheep antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays. Anti-Sheep IgG (H&L) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.