Rabbit anti-Cat IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

Catalog No. ABIN1044009

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG and Cat Serum.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Cat IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Comment:

Texas Red™ is a registered trademark of Molecular Probes Inc.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Centrifuge product if not completely clear after standing at room temperature.
Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit F(ab')2 Anti-Cat Texas Red™ Conjugated Antibody, Rabbit Fab2 Anti-Cat Antibody Texas Red™ Conjugation, Rabbit Fab'2 Anti-Cat Antibody Texas Red™ Conjugation

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.