Goat anti-Mouse IgG2a (Heavy Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN1118479

Product Details

Target: IgG2a

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Characteristics: Mouse IgG2a secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment compositions.

Purification: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein

Isotype: IgG2a

Application Details

Application Notes: Anti-Mouse IgG2a Peroxidase Secondary antibodies are suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG2a

Abstract: IgG2a Products

Target Type: Antibody

Background: Synonyms: IgG2a antibody, mouse IgG2a, anti-subclass secondary antibody, anti mouse IgG Peroxidase Conjugated, mouse secondary antibody