Goat anti-Mouse IgG2a (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN1118481

Product Details

Target: IgG2a

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Cross-Reactivity: Mouse (Murine)

Characteristics: Mouse IgG2a secondary antibody is available in a variety of formats. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG2a secondary antibody is available in a variety of formats. Anti-Mouse IgG2a biotin conjugated secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge product if not completely clear after standing at room temperature.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Jia, Xu, Smeets, Buhl, Moeller, Floege, Klinkhammer, Boor: "The Role of Platelet-Derived Growth Factor in Focal Segmental Glomerulosclerosis." in: Journal of the American Society of Nephrology : JASN, Vol. 34, Issue 2, pp. 241-257, (2023) (PubMed).

Target Details

Target: IgG2a

Abstract: IgG2a Products

Target Type: Antibody

Background:

Synonyms: goat anti-MOUSE IgG2a Antibody biotin Conjugation, goat anti-Mouse IgG2a (gamma 2a) biotin Conjugated Antibody

Background: Anti-Mouse IgG2a Biotin Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.