Goat anti-Mouse IgA, IgG, IgM (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN129740

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG IgA IgM

Cross-Reactivity: Mouse (Murine)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG IgA and IgM whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Mouse IgG IgA IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:200,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG IgA IgM (IgA, IgG, IgM Products)

Target Type: Antibody

Background:

Synonyms: Goat Anti Mouse IgG IgA IgM Pre-Adsorbed, Goat Anti-Mouse IgG IgA IgM Antibody, Goat Anti-Mouse IgGAM

Background: Anti-Mouse IgG IgA IgM Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.