Mouse anti-Rat Ig (Chain kappa) Antibody (FITC)

Catalog No. ABIN133283

Product Details

Target: Ig

Binding Specificity: Chain kappa

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Specificity: Rat Kappa Chain

Characteristics: anti-rat Ig chain monoclonal antibody recognizes a determinant on rat kappa chains and binds preferentially to the Ig-1b allotype. This antibody provides a suitable marker for virtually all immunoglobin, regardless of class since 5 % or less of rat light chains are lambda. ABIN133283 detects surface immunoglobulin of rat peripheral blood B lymphocytes and has been used in indirect binding assays for detecting rat antibodies. Applications include flow cytometry for directly labelling rat B lymphocytes and as an excellent specific secondary antibody for use with rat monoclonals.

Purification: Purified from ascites via Protein G Chromatography

Immunogen: Pooled rat serum IgG . Fusion Partner: P3-NSI-1-Ag4 (NS1/1) . Donor: BALB/c spleen

Clone: OX-12

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C. For long term storage aliquot and freeze unused portion at -20C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Target Details

Target: Ig

Abstract: Ig Products