Pig anti-Human IgE (Fc Region) Antibody (HRP)

Catalog No. ABIN2622607

Product Details

Target: IgE

Binding Specificity: Fc Region

Reactivity: Human

Host: Pig

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunodiffusion (ID), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human IgE, Fc specific.

Purification: Purified IgG

Immunogen: Purified monoclonal IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA, ICC, ID, IHC, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgE in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions may vary widely: for histochemical and cytochemical use usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgE

Abstract: IgE Products