Rabbit anti-Dog IgG (F(ab')2 Region) Antibody (Biotin)

Catalog No. ABIN299855

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Specificity: Anti-biotin, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum. No reaction was observed against Dog IgG F(c)

Purification: Immunoaffinity purified

Immunogen: Dog IgG F(ab')2 fragment.

Application Details

Application Notes: Approved: ELISA (1:15000 - 1:65000), IF, IHC, WB

Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Canine IgG Fab'2