Goat anti-Mouse IgG2b Antibody (Cy5) - Preadsorbed

Catalog No. ABIN376049

Quick Overview for Goat anti-Mouse IgG2b Antibody (Cy5) - Preadsorbed (ABIN376049)

Target: IgG2b

Clonality: Polyclonal

Conjugate: Cy5

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), FLISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG2b, Human ads-CY5

Specificity: Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG2b-CY5 antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Purification:

Purified by affinity chromatography on mouse IgG2b covalently linked to agarose, cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG2b

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Nelson, Nonnecke, Reinhardt, Waters, Beitz, Lippolis: "Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3." in: PLoS ONE, Vol. 6, Issue 6, pp. e21674, (2011) (PubMed).

Hardie, Baldwin, Naylor, Haworth, Hou, Lax, Curnow, Willcox, MacFadyen, Isacke, Buckley: "The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation." in: Immunology, Vol. 133, Issue 3, pp. 288-95, (2011) (PubMed).

Parsonage, Filer, Bik, Hardie, Lax, Howlett, Church, Raza, Wong, Trebilcock, Scheel-Toellner, Salmon, Lord, Buckley: "Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha." in: Arthritis research & therapy, Vol. 10, Issue 2, pp. R47, (2008) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG2b, Human ads-CY5

NCBI_Official_Symbol: Ighg2b

Gene ID: 16016

UniProt: P01867