Goat anti-Mouse IgG1 (Heavy Chain) Antibody (HRP)

Catalog No. ABIN376248

Quick Overview for Goat anti-Mouse IgG1 (Heavy Chain) Antibody (HRP) (ABIN376248)

Target: IgG1

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG1 as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry to conform to characteristics of a standard reference reagent.

Purification: Affinity chromatography on mouse IgG1 covalently linked to agarose. Cross Absorption: Mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.

Application Details

Application Notes: Working Dilution:
ELISA: 1:4,000-1:8,000
Indirect immunostaining in conjunction with mouse IgG1 monoclonal antibodies.
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C or long-term at -20 °C.

References

Margry, Kersemakers, Hoek, Arkesteijn, Wieland, van Eden, Broere: "Activated peritoneal cavity B-1a cells possess regulatory B cell properties." in: PLoS ONE, Vol. 9, Issue 2, pp. e88869, (2014) (PubMed).

Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody