Rat anti-Mouse IgG1 Antibody (HRP)

Catalog No. ABIN376256

Quick Overview for Rat anti-Mouse IgG1 Antibody (HRP) (ABIN376256)

Target: IgG1

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA

Clone: SB77E

Isotype: IgG2b

Product Details

Reactivity: Mouse

Host: Rat

Specificity: Reacts with Mouse IgG1 as demonstrated by ELISA.

Characteristics: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or Flow Cytometry to conform to the characteristics of a standard reference reagent.

Purification: Affinity chromatography on pooled mouse Igs with kappa light chains covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
ELISA: 1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C or long-term at -20 °C.

References

Joshi, Adamcakova-Dodd, Jing, Wongrakpanich, Gibson-Corley, Thorne, Salem: "Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy." in: The AAPS journal, (2014) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody