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Goat anti-Mouse IgG1 (Heavy Chain) Antibody

ELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Catalog No. ABIN376864

Quick Overview for Goat anti-Mouse IgG1 (Heavy Chain) Antibody (ABIN376864)

Target

IgG1

Clonality

  • 93
  • 52
Polyclonal

Application

  • 85
  • 58
  • 50
  • 44
  • 32
  • 20
  • 19
  • 14
  • 14
  • 9
  • 9
  • 9
  • 8
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Isotype

IgG
  • Binding Specificity

    • 40
    • 18
    • 9
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain

    Reactivity

    • 97
    • 23
    • 15
    • 9
    • 7
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    Mouse

    Host

    • 63
    • 40
    • 31
    • 15
    • 10
    • 6
    • 1
    Goat

    Specificity

    Reacts with the heavy chain of mouse IgG 1 as demonstrated by ELISA and flow cytometry.

    Characteristics

    Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins.
    To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry to conform to characteristics of a standard reference reagent.

    Purification

    Purified
  • Application Notes

    Working Dilution:
    <= 1 µg/10^6 cells
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Buffer

    1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2.

    Preservative

    Azide free

    Handling Advice

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C
  • Harada, Tanaka, Motomura, Harada, Ohno, Ohno, Yanagi, Inoue, Kubo: "The 3' Enhancer CNS2 Is a Critical Regulator of Interleukin-4-Mediated Humoral Immunity in Follicular Helper T Cells." in: Immunity, Vol. 36, Issue 2, pp. 188-200, (2012) (PubMed).

  • Target

    IgG1

    Target Type

    Antibody
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