Rat anti-Mouse IgG1 Antibody

Catalog No. ABIN376877

Quick Overview for Rat anti-Mouse IgG1 Antibody (ABIN376877)

Target: IgG1

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Clone: SB77e

Isotype: IgG2b

Product Details

Reactivity: Mouse

Host: Rat

Specificity: Mouse IgG1 Fc Minimal reactivity to human, goat, and rabbit IgG

Characteristics: Rat Anti-Mouse IgG1-UNLB

Purification: Purified

Immunogen: Unknown

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , WB - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1.0 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Kim, Kim, Park, Park: "TRIF Deficiency does not Affect Severity of Ovalbumin-induced Airway Inflammation in Mice." in: Immune network, Vol. 14, Issue 5, pp. 249-54, (2014) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody