Pig anti-Horse kappa/lambda Light Chain (Kappa/Lambda-IgLC) (Free & Bound) Antibody
Kappa/Lambda-IgLC
IP
Polyclonal
unconjugated
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- Target See all kappa/lambda Light Chain (Kappa/Lambda-IgLC) products
- kappa/lambda Light Chain (Kappa/Lambda-IgLC)
- Binding Specificity
- Free & Bound
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Reactivity
- Horse
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Host
- Pig
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Clonality
- Polyclonal
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Application
- Immunoprecipitation (IP)
- Specificity
- Precipitating polyclonal Swine antiSerum to Horse light chains, free and bound.
- Cross-Reactivity (Details)
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, but a wide scale of reactivity with IgG of other mammalian species must be assumed. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentRations of the reactants and the sensitivity of the assay format.
- Characteristics
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As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal horse serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types, also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As precipitating antiserum in a variety of immunodiffusion techniques including immunoelectrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
Clone: Polyclonal - Purification
- Delipidated, heat inactivated lyophilized stable whole serum
- Sterility
- Heat-inactivated
- Immunogen
- Purified equine light chains isolated from polyclonal horse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- Application Notes
- In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single immunodiffusion, use 1 % antiserum in agar gel to establish a standard curve.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled swine serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.
- Buffer
- Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled swine serum. No foreign proteins added.
- Preservative
- Without preservative
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- kappa/lambda Light Chain (Kappa/Lambda-IgLC)
- Alternative Name
- kappa lambda (Kappa/Lambda-IgLC Products)
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