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Goat anti-Goat IgA (Heavy Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN5608139
  • Target See all IgA products
    IgA
    Binding Specificity
    • 104
    • 60
    • 38
    • 15
    • 15
    • 13
    • 8
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 161
    • 58
    • 41
    • 16
    • 16
    • 14
    • 14
    • 13
    • 13
    • 12
    • 10
    • 7
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Rat, Goat
    Host
    • 200
    • 98
    • 48
    • 17
    • 12
    • 6
    • 2
    Goat
    Clonality
    • 307
    • 61
    Polyclonal
    Application
    • 179
    • 136
    • 129
    • 111
    • 100
    • 97
    • 56
    • 40
    • 39
    • 29
    • 28
    • 24
    • 23
    • 18
    • 17
    • 17
    • 17
    • 7
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Characteristics
    Rat IgA antibodies detects the alpah chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Rat IgA alpha heavy chain

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: Anti-Rat IgA antibody is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target
    IgA
    Abstract
    IgA Products
    Target Type
    Antibody
    Background

    Synonyms: goat anti-Rat IgA Antibody, Immunoglobulin, Rat IgA

    Background: Rat IgA antibodies detects the alpah chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.

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