Mouse anti-Rabbit IgG (Light Chain) Antibody (HRP)

Catalog No. ABIN7205197

Product Details

Target: IgG

Binding Specificity: Light Chain

Reactivity: Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: HRP, Mouse Anti-Rabbit IgG LCS

Specificity: The antibody reacts with kappa light chains on rabbit IgG, while it doesn't react with the heavy chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins

Characteristics: HRP conjugated Mouse Anti-Rabbit IgG light chain specific secondary antibody,Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.,Mouse Anti-Rabbit IgG

Purification: Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally > 95% purity based on SDS-PAGE

Immunogen: Rabbit IgG light chain

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000).

Comment:

50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. This product solve these problems through less background noise, and enhanced accuracy. Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.4, containing 1 % BSA as stablizer and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody