Recombinant Alpaca anti-Mouse IgG1 Antibody (Atto 488)

Catalog No. ABIN7272921

Quick Overview for Recombinant Alpaca anti-Mouse IgG1 Antibody (Atto 488) (ABIN7272921)

Target: IgG1

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 10A4

Product Details

Reactivity: Mouse

Host: Alpaca

Expression System: E.coli

Specificity: Mouse IgG1, does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Immunogen: FluoTag-X2 anti-Mouse IgG1 is a species-specific FluoTag directed against IgG1 from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Storage: -20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgG1

Target Type: Antibody

Background: In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.