Recombinant Alpaca anti-Mouse IgG1 Antibody (DBCO)

Catalog No. ABIN7272945

Quick Overview for Recombinant Alpaca anti-Mouse IgG1 Antibody (DBCO) (ABIN7272945)

Target: IgG1

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: DBCO

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 10A4

Product Details

Reactivity: Mouse

Host: Alpaca

Expression System: E.coli

Specificity: Mouse IgG1, does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Immunogen: sdAb anti-Mouse is a species-specific sdAb directed against mouse IgG1. anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgG1

Target Type: Antibody

Background: Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either lambda or kappa light chain.