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|Antigen||Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-RPS6KB1 AntibodyTarget Details RPS6KB1 Application Details Handling Images
|Specificity||This antibody detects RPS6KB1 at C-term.|
Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide from the C Terminus of the protein sequence according to NP_003152.1 Genename: RPS6KB1|
Target Details RPS6KB1Product Details anti-RPS6KB1 Antibody Application Details Handling Images back to top
|Alternative Name||RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)|
|Background||The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser-434. In Alzheimer's Disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1|
|Molecular Weight||60kDa according to NP_003152.1|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma|
Application DetailsProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling Images back to top
Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-1 μg/mL. Detects a band of Approx 65-70 kDa in Human Skeletal Muscle and Placenta lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|