Speedy Homolog B (Xenopus Laevis) (SPDYB) (AA 1-268) protein (His tag)
custom-made
SPDYB
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- Speedy Homolog B (Xenopus Laevis) (SPDYB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-268
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Predefined custom protein recombinant Spdye4a Protein expressed in mammalian cells.
- Sequence
- MGEGTPGVDS ARVQEEGGRD QSLGFVEGRI QVGRIVTAGQ LSLCSEEQSP QPGITRPSPG VVVDGESSGL AEPRVEATPQ PPSSIQKRKR DESLDSEDDL AELFEPDPQP VWSVEMLCGL RMRLKRRRVS TVRPEHHKVF TKLLEDPVVK KFLTWDKMLR VSDKYLLSMV IAYFSRAGLF SWQYRPIHFF LALYLANDME EDNQAPKQDI FYFLYGKSYA QRPMFHKLRF QFIRSMGWKI WVSREECEEI QAYNPDLWVW ARDRTNLT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Speedy Homolog B (Xenopus Laevis) (SPDYB)
- Alternative Name
- Spdye4a
- Background
- Speedy protein E4A (Rapid inducer of G2/M progression in oocytes E4A) (RINGO E4A) (mSpy/Ringo E4A),FUNCTION: Promotes progression through the cell cycle via binding and activation of CDK1. {ECO:0000269|PubMed:15611625}.
- Molecular Weight
- 31.0 kDa
- UniProt
- Q5IBH6
- AlphaFold
- Q5IBH6
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