anti-Mouse (Murine) CHRM3 antibody for Western Blotting

Recommended CHRM3 Antibody (supplied by: Log in to see )

Antigen
Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies
  • EGBRS
  • HM3
  • MACHR
  • Chrm-3
  • M3
  • M3R
  • cholinergic receptor muscarinic 3
  • cholinergic receptor, muscarinic 3
  • cholinergic receptor, muscarinic 3, cardiac
  • CHRM3
  • Chrm3
Epitope
AA 461-479, Intracellular
17
15
15
8
8
6
4
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
93
32
29
14
6
6
6
6
6
6
6
6
5
5
4
3
3
2
1
Host
Rabbit
107
6
Clonality
Polyclonal
Conjugate
This CHRM3 antibody is un-conjugated
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
50
43
30
29
13
4
4
4
2
1
1
1
Options
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Catalog No. ABIN1742045
$ 832.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2075982 ABIN674484 IF (p) IHC (p) WB Rabbit IgG AA 546-589 Log in to see Polyclonal 3
3.2075982 ABIN2736526 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN6257058 ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN5861239 WB Rabbit Log in to see Polyclonal 0
3.2075982 ABIN3048209 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN674493 IHC (p) WB HRP Rabbit IgG AA 546-589 Log in to see Polyclonal 0
1 ABIN674486 IHC (p) WB Biotin Rabbit IgG AA 546-589 Log in to see Polyclonal 0
1 ABIN6029810 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0
1 ABIN6029808 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies
Epitope AA 461-479, Intracellular
(17), (15), (15), (8), (8), (6), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(93), (32), (29), (14), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (2), (1)
Host Rabbit
(107), (6)
Clonality
Polyclonal
Conjugate This CHRM3 antibody is un-conjugated
(3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(50), (43), (30), (29), (13), (4), (4), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CHRM3 Antibody

Target Details CHRM3 Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) Images
Sequence (C)TLAKRFALKT RSQITKRKR
Cross-Reactivity (Details) Human, mouse, bovine, pig - identical.
Characteristics Peptide Confirmation:
Confirmed by amino acid analysis and massspectrography.

Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.

Antibody concentration after reconstitution:
0.8 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Purification Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino residues 461-479 of rat m3. 3rd intracellular loop.
Plasmids, Primers & others

Target Details CHRM3

Product Details anti-CHRM3 Antibody Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) Images back to top
Antigen
Alternative Name M3 Muscarinic Receptor (CHRM3 Antibody Abstract)
Background Synonyms:
CHRM3

The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5.1-2 The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.-2 The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscls. Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor contros. They also participate in learning and memory processins.-5 The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves.-7 m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholins. m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues. Involvement of muscarinic receptors in apoptosis and cell growth has been reportes.0-14 m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.0 Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, uncleas.1-14 In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.1-14 It has been designed to recognize m3 muscarinic receptor human, mouse and rat samples .
Gene ID 24260
UniProt P08483
Pathways Synaptic Membrane

Application Details

Product Details anti-CHRM3 Antibody Target Details CHRM3 Handling References for anti-CHRM3 antibody (ABIN1742045) Images back to top
Application Notes Recommended Dilution:
WB: approx. 1:200
Restrictions For Research Use only

Handling

Product Details anti-CHRM3 Antibody Target Details CHRM3 Application Details References for anti-CHRM3 antibody (ABIN1742045) Images back to top
Format Lyophilized
Reconstitution 0.2 mL deionized water.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Storage 4 °C/-20 °C
Storage Comment The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

References for anti-CHRM3 antibody (ABIN1742045)

Product Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Handling Images back to top
Background publications

Ukegawa, Takeuchi, Kusayanagi, Mitamura: "Growth-promoting effect of muscarinic acetylcholine receptors in colon cancer cells." in: Journal of cancer research and clinical oncology, Vol. 129, Issue 5, pp. 272-8, 2003

Forsythe, Kogut, McConville, Fu, McCauley, Halayko, Liu, Kao, Fernandes, Bellam, Fuchs, Sinha, Bell, Camoretti-Mercado, Solway: "Structure and transcription of the human m3 muscarinic receptor gene." in: American journal of respiratory cell and molecular biology, Vol. 26, Issue 3, pp. 298-305, 2002

Frucht, Jensen, Dexter, Yang, Xiao: "Human colon cancer cell proliferation mediated by the M3 muscarinic cholinergic receptor." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 9, pp. 2532-9, 1999

Images

Product Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) back to top
Supplier Images
Western Blotting (WB) image for anti-Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 461-479), (Intracellular) antibody (ABIN1742045) Western blot analysis of rat brain lysate: 1. Anti-M3 Muscarinic Receptor antibody, (...