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|Antigen||Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies|
|Epitope||AA 461-479, Intracellular Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CHRM3 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CHRM3 AntibodyTarget Details CHRM3 Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) Images
|Cross-Reactivity (Details)||Human, mouse, bovine, pig - identical.|
Confirmed by amino acid analysis and massspectrography.
Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.
Antibody concentration after reconstitution:
1 µg peptide per 1 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino residues 461-479 of rat m3. 3rd intracellular loop.|
|Plasmids, Primers & others|
Target Details CHRM3Product Details anti-CHRM3 Antibody Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) Images back to top
|Alternative Name||M3 Muscarinic Receptor (CHRM3 Antibody Abstract)|
The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5.1-2 The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.-2 The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscls. Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor contros. They also participate in learning and memory processins.-5 The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves.-7 m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholins. m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues. Involvement of muscarinic receptors in apoptosis and cell growth has been reportes.0-14 m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.0 Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, uncleas.1-14 In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.1-14 It has been designed to recognize m3 muscarinic receptor human, mouse and rat samples .
Application DetailsProduct Details anti-CHRM3 Antibody Target Details CHRM3 Handling References for anti-CHRM3 antibody (ABIN1742045) Images back to top
WB: approx. 1:200
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHRM3 Antibody Target Details CHRM3 Application Details References for anti-CHRM3 antibody (ABIN1742045) Images back to top
|Reconstitution||0.2 mL deionized water.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).|
References for anti-CHRM3 antibody (ABIN1742045)Product Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Handling Images back to top
Ukegawa, Takeuchi, Kusayanagi, Mitamura: "Growth-promoting effect of muscarinic acetylcholine receptors in colon cancer cells." in: Journal of cancer research and clinical oncology, Vol. 129, Issue 5, pp. 272-8, 2003
Forsythe, Kogut, McConville, Fu, McCauley, Halayko, Liu, Kao, Fernandes, Bellam, Fuchs, Sinha, Bell, Camoretti-Mercado, Solway: "Structure and transcription of the human m3 muscarinic receptor gene." in: American journal of respiratory cell and molecular biology, Vol. 26, Issue 3, pp. 298-305, 2002
Frucht, Jensen, Dexter, Yang, Xiao: "Human colon cancer cell proliferation mediated by the M3 muscarinic cholinergic receptor." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 9, pp. 2532-9, 1999
ImagesProduct Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Handling References for anti-CHRM3 antibody (ABIN1742045) back to top