|Antigen||Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies|
|Epitope||AA 580-589 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This CHRM3 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-CHRM3 AntibodyTarget Details CHRM3 Application Details Handling Images
|Specificity||Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.|
|Immunogen||Synthetic peptide M3 (580-589) covalently attached to a carrier protein.|
|Plasmids, Primers & others|
Target Details CHRM3Product Details anti-CHRM3 Antibody Application Details Handling Images back to top
|Alternative Name||Muscarinic Acetylcholine Receptor M3 (CHRM3 Antibody Abstract)|
|Background||This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3|
Application DetailsProduct Details anti-CHRM3 Antibody Target Details CHRM3 Handling Images back to top
Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Images back to top
|Reconstitution||Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.|
|Precaution of Use||Do Not Use Sodium Azide as preservative.|
|Handling Advice||Avoid repeated freezing and thawing. Dilute only prior to immediate use|
|Storage||4 °C/-20 °C|
|Storage Comment||Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.|