anti-Rat (Rattus) CHRM3 antibody for Immunohistochemistry (Frozen Sections)

Recommended CHRM3 Antibody (supplied by: Log in to see )

Antigen
Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies
  • EGBRS
  • HM3
  • MACHR
  • Chrm-3
  • M3
  • M3R
  • cholinergic receptor muscarinic 3
  • cholinergic receptor, muscarinic 3
  • cholinergic receptor, muscarinic 3, cardiac
  • CHRM3
  • Chrm3
Epitope
AA 580-589
17
15
15
8
8
5
4
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
93
33
29
14
6
6
6
6
6
6
6
6
5
5
4
3
3
2
1
Host
Rabbit
107
6
Clonality
Polyclonal
Conjugate
This CHRM3 antibody is un-conjugated
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
50
43
30
29
13
4
4
4
2
1
1
Supplier
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Catalog No. ABIN112905
$ 544.50
Plus shipping costs $45.00

General

Antigen Cholinergic Receptor, Muscarinic 3 (CHRM3) Antibodies
Epitope AA 580-589
(17), (15), (15), (8), (8), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(93), (33), (29), (14), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (2), (1)
Host Rabbit
(107), (6)
Clonality
Polyclonal
Conjugate This CHRM3 antibody is un-conjugated
(3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(50), (43), (30), (29), (13), (4), (4), (4), (2), (1), (1)
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Product Details anti-CHRM3 Antibody

Target Details CHRM3 Application Details Handling Images
Specificity Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.
Immunogen Synthetic peptide M3 (580-589) covalently attached to a carrier protein.
Plasmids, Primers & others

Target Details CHRM3

Product Details anti-CHRM3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Muscarinic Acetylcholine Receptor M3 (CHRM3 Antibody Abstract)
Background This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3
Gene ID 1131
NCBI Accession NP_000731
UniProt P20309
Pathways Synaptic Membrane

Application Details

Product Details anti-CHRM3 Antibody Target Details CHRM3 Handling Images back to top
Application Notes Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHRM3 Antibody Target Details CHRM3 Application Details Images back to top
Format Liquid
Reconstitution Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.
Preservative Azide free
Precaution of Use Do Not Use Sodium Azide as preservative.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.