anti-Human CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • CD3-ZETA
  • CD3H
  • CD3Q
  • CD3Z
  • T3Z
  • TCRZ
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • TCRzeta
  • CD247 molecule
  • CD247 antigen
  • CD3zeta-2
  • CD3zeta-1
  • Cd247 molecule
  • CD247
  • Cd247
  • cd3z
  • cd247
Reactivity
Human
222
148
36
7
4
4
1
1
1
1
Host
Mouse
103
99
19
15
12
5
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
17
11
10
8
7
6
6
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
194
143
99
37
35
29
19
17
5
4
3
3
2
1
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Catalog No. ABIN1724734
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.87045 ABIN931487 FACS IF ELISA WB Mouse IgG1 Log in to see 4B10 0
23.37045 ABIN2749060 ICC FACS WB Mouse IgG2b pTyr72 Log in to see EM-26 1
19.735905 ABIN514136 PLA WB Rabbit AA 1-164 Log in to see Polyclonal 0
19.735905 ABIN560227 IHC (p) IP ELISA WB Mouse IgG1 kappa AA 1-164 Log in to see 4A12-F6 0
18.87045 ABIN3022813 IHC WB Rabbit IgG Log in to see Polyclonal 0
16.735905 ABIN782349 EIA IHC (p) IP WB Mouse IgG1 AA 1-165 Log in to see 4A12-F6 0
16.735905 ABIN1845770 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see 0
16.634544 ABIN965779 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4B10 4
15.870449 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17 0
15.870449 ABIN2749058 FACS WB Mouse IgG1 pTyr142 Log in to see EM-54 0
15.870449 ABIN2749059 FACS WB Mouse IgG1 pTyr111, pTyr123 Log in to see EM-55 0
13.735905 ABIN2869487 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 4B10 0
13.735905 ABIN5574275 ELISA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4B10 0
13.735905 ABIN1106129 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4B10 2
12.235905 ABIN543455 ELISA WB Rabbit Log in to see Polyclonal 3
12.134544 ABIN5539696 ELISA WB Goat Internal Region Log in to see Polyclonal 0
12.134544 ABIN4901197 ELISA WB Rabbit IgG AA 51-100 Log in to see Polyclonal 0
12.134544 ABIN3181950 ELISA IHC WB Rabbit IgG pTyr142 Log in to see Polyclonal 0
12.134544 ABIN3183756 ELISA IHC WB Rabbit IgG Ser212 Log in to see Polyclonal 0
12.134544 ABIN4901189 ELISA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4B10 0

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human
(222), (148), (36), (7), (4), (4), (1), (1), (1), (1)
Host Mouse
(103), (99), (19), (15), (12), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(17), (11), (10), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(194), (143), (99), (37), (35), (29), (19), (17), (5), (4), (3), (3), (2), (1)
Pubmed 3 references available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN1724734) Images
Immunogen Purified recombinant fragment of human CD247 expressed in E. Coli.
Clone 4B10
Isotype IgG1
Plasmids, Primers & others

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 antibody (ABIN1724734) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Weight 18 kDa
Gene ID 919
HGNC 919
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 antibody (ABIN1724734) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 antibody (ABIN1724734) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-CD247 antibody (ABIN1724734)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Nambiar, Fisher, Warke, Krishnan, Mitchell, Delaney, Tsokos: "Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and augments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 1948-55, 2003

Dumont, Blanchard, Di Bartolo, Lezot, Dufour, Jauliac, Hivroz: "TCR/CD3 down-modulation and zeta degradation are regulated by ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1705-12, 2002

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN1724734) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD247 Molecule (CD247) antibody (ABIN1724734) anti-CD247 Molecule (CD247) antibody
Immunocytochemistry (ICC) image for anti-CD247 Molecule (CD247) antibody (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD247 Molecule (CD247) antibody (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 3)
Western Blotting (WB) image for anti-CD247 Molecule (CD247) antibody (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 4)