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|Antigen||Toll-Like Receptor 4 (TLR4) Antibodies|
|Reactivity||Dog (Canine), Human Alternatives|
|Conjugate||This TLR4 antibody is un-conjugated Alternatives|
Blocking Peptide (BP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), In vitro Assay (in vitro), Neutralization (Neut)
|45 references available|
|Supplier||Log in to see|
Product Details anti-TLR4 AntibodyTarget Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN4360113) Images
|Specificity||The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).|
|Purification||Protein G purified|
|Immunogen||This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.|
|Plasmids, Primers & others|
Target Details TLR4Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN4360113) Images back to top
|Alternative Name||TLR4 (TLR4 Antibody Abstract)|
|Background||Gene Symbol: TLR4|
|Pathways||TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN4360113) Images back to top
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Functional, In vitro assay, Blocking/Neutralizing, CyTOF-readyConfocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please see publications. Flow (Cell Surface): 2-5 μg/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details Use in vitro assays reported in scientific literature (PMID 24676500) Use in Functional reported in scientific literature (PMID 25412776). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27744428)
Not for use in: Western Blot
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN4360113) Images back to top
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-TLR4 antibody (ABIN4360113)Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
|Product cited in:||
Larsson, Tengroth, Xu, Uddman, Kumlien Georén, Cardell: "Substance P represents a novel first-line defense mechanism in the nose." in: The Journal of allergy and clinical immunology, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).
Heftrig, Sturm, Oppermann, Kontradowitz, Jurida, Schimunek, Woschek, Marzi, Relja: "Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients." in: Mediators of inflammation, Vol. 2017, pp. 2608349, 2017
Li, Song, Ao, Reece, Cleveland, Dong, Fullerton, Meng: "ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 7, pp. 1339-1351, 2017 Method employed by authors: Neutralization (Neut), Western Blotting (WB) (Sample species: Human).
Pérez-Figueroa, Torres, Sánchez-Zauco, Contreras-Ramos, Alvarez-Arellano, Maldonado-Bernal: "Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection." in: Innate immunity, Vol. 22, Issue 2, pp. 103-12, 2016 (Sample species: Human). Further details: In vitro Assay,Blocking Peptide,Neutralization
Kopp, Kupsch, Schromm: "Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling." in: Biochimica et biophysica acta, Vol. 1863, Issue 4, pp. 660-72, 2016 (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence
Schmid, Novacek, Vogelsang, Papay, Primas, Eser, Panzer: "Platelets Toll-like receptor-4 in Crohns disease." in: European journal of clinical investigation, 2016
Imai, Fujita, Kajiya, Ouhara, Yoshimoto, Matsuda, Takeda, Kurihara: "Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 5, pp. 1777-1786, 2016
Moreira, Costa-Pereira, Alves, Marteleto, Ribeiro, Peruhype-Magalhães, Giunchetti, Martins-Filho, Araújo: "Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes." in: Veterinary parasitology, Vol. 220, pp. 33-45, 2016 Method employed by authors: Flow Cytometry (FACS)
Song, Fullerton, Ao, Zheng, Zhao, Meng: "BMP-2 and TGF-β1 mediate biglycan-induced pro-osteogenic reprogramming in aortic valve interstitial cells." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 4, pp. 403-12, 2015 (Sample species: Human). Further details: Functional Studies,Blocking Peptide,Neutralization
Jamin, Dehoux, Dossier, Fila, Heming, Monteiro, Deschênes: "Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome." in: Clinical and experimental immunology, Vol. 182, Issue 3, pp. 332-45, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Youn, Shim, Lee, Ji, Oh, Hong, Lee, Park, Lee, Choi, Chung, Kang: "Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis." in: Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, Vol. 23, Issue 3, pp. 139-44, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Feng, Feng, Xing, Shen, Tan, Huang, Lu, Tao, Zhang, Li, Gu: "Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs)." in: Cell and tissue research, Vol. 356, Issue 2, pp. 369-80, 2014 (Sample species: Human). Further details: In vitro Assay,Blocking Peptide,Neutralization
Komine-Aizawa, Hirohata, Aizawa, Abiko, Hayakawa: "Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine." in: Placenta, Vol. 36, Issue 1, pp. 27-33, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Rojas-Bernabé, Garcia-Hernández, Maldonado-Bernal, Delegado-Dominguez, Ortega, Gutiérrez-Kobeh, Becker, Aguirre-Garcia: "Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4." in: Parasitology, Vol. 141, Issue 6, pp. 788-800, 2014 (Sample species: Human).
Käbisch, Mejías-Luque, Gerhard, Prinz: "Involvement of Toll-like receptors on Helicobacter pylori-induced immunity." in: PLoS ONE, Vol. 9, Issue 8, pp. e104804, 2014 Method employed by authors: Neutralization (Neut) (Sample species: Human).
Yuan, Zhou, Wang, Li, Xie, Zhao, Xu, Shen: "Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production." in: Cell death & disease, Vol. 4, pp. e794, 2013 (Sample species: Human).
Harman, Bye, Nasr, Sandgren, Kim, Mercier, Botting, Lewin, Cunningham, Cameron: "Identification of lineage relationships and novel markers of blood and skin human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 1, pp. 66-79, 2012
Wu, Chi, Hsieh, Luo, Yang: "TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2." in: Journal of cellular physiology, Vol. 223, Issue 2, pp. 480-91, 2010
Pillay, Ramakers, Kamp, Loi, Lam, Hietbrink, Leenen, Tool, Pickkers, Koenderman: "Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia." in: Journal of leukocyte biology, Vol. 88, Issue 1, pp. 211-20, 2010
Matsunaga, Tsuchimori, Matsumoto, Ii: "TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules." in: Molecular pharmacology, Vol. 79, Issue 1, pp. 34-41, 2010
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