anti-Dog (Canine) TLR4 antibody for Flow Cytometry

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Reactivity
Dog (Canine), Human
564
272
174
92
71
71
63
26
12
9
4
2
1
1
Host
Mouse
349
273
46
8
Clonality
Monoclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
45
23
15
14
13
10
8
8
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), In vitro Assay (in vitro), Neutralization (Neut)
394
353
190
135
129
121
102
91
34
31
27
26
25
21
19
15
13
9
7
4
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4360113
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.325633 ABIN2479746 FACS IP WB Mouse IgG2a Log in to see HTA125 4
9.825633 ABIN119330 Func FACS IP WB Mouse IgG2a Log in to see HTA125 2
9.483948 ABIN252065 FACS FITC Mouse IgG2a Log in to see HTA125 24
7 ABIN959964 FACS PE Mouse IgG2a, kappa Log in to see HTA125 0
7 ABIN956803 FACS FITC Mouse IgG2a, kappa Log in to see HTA125 0
5.5 ABIN2479755 Func FACS IP WB Mouse IgG2a Log in to see HTA125 4
5.5 ABIN2479752 FACS Biotin Mouse IgG2a Log in to see HTA125 4
5.5 ABIN2479753 FACS FITC Mouse IgG2a Log in to see HTA125 4
5.5 ABIN2479757 FACS PE Mouse IgG2a Log in to see HTA125 4
5.5 ABIN2479750 FACS Alexa Fluor 647 Mouse IgG2a Log in to see HTA125 4
5.5 ABIN2479748 FACS Alexa Fluor 488 Mouse IgG2a Log in to see HTA125 4
4 ABIN6181202 FACS IF IHC (f) APC Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181209 FACS IF IHC (f) PerCP Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181210 FACS IF IHC (f) PE Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN6181213 FACS IF IHC (f) Mouse IgG2a, kappa Log in to see TLR4-230 0
4 ABIN119329 FACS FITC Mouse IgG2a Log in to see HTA125 2
4 ABIN119333 FACS PE Mouse IgG2a Log in to see HTA125 2
4 ABIN119331 FACS IP WB Mouse IgG2a Log in to see HTA125 2
1.9839478 ABIN4964343 FACS Func IA ICC IF IP WB Mouse IgG2a Log in to see HTA1245 0
1 ABIN119332 FACS IP WB Mouse IgG2a Log in to see HTA125 9

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Reactivity Dog (Canine), Human
(564), (272), (174), (92), (71), (71), (63), (26), (12), (9), (4), (2), (1), (1)
Host Mouse
(349), (273), (46), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (45), (23), (15), (14), (13), (10), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Peptide (BP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), In vitro Assay (in vitro), Neutralization (Neut)
(394), (353), (190), (135), (129), (121), (102), (91), (34), (31), (27), (26), (25), (21), (19), (15), (13), (9), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 45 references available
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN4360113) Images
Specificity The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Purification Protein G purified
Immunogen This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Clone HTA125
Isotype IgG2a
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN4360113) Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background Gene Symbol: TLR4
Gene ID 7099
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN4360113) Images back to top
Application Notes Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Functional, In vitro assay, Blocking/Neutralizing, CyTOF-readyConfocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please see publications. Flow (Cell Surface): 2-5 μg/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details Use in vitro assays reported in scientific literature (PMID 24676500) Use in Functional reported in scientific literature (PMID 25412776). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27744428)

Not for use in: Western Blot
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN4360113) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TLR4 antibody (ABIN4360113)

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
Product cited in:

Larsson, Tengroth, Xu, Uddman, Kumlien Georén, Cardell: "Substance P represents a novel first-line defense mechanism in the nose." in: The Journal of allergy and clinical immunology, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Heftrig, Sturm, Oppermann, Kontradowitz, Jurida, Schimunek, Woschek, Marzi, Relja: "Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients." in: Mediators of inflammation, Vol. 2017, pp. 2608349, 2017

Li, Song, Ao, Reece, Cleveland, Dong, Fullerton, Meng: "ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 7, pp. 1339-1351, 2017 Method employed by authors: Neutralization (Neut), Western Blotting (WB) (Sample species: Human).

Pérez-Figueroa, Torres, Sánchez-Zauco, Contreras-Ramos, Alvarez-Arellano, Maldonado-Bernal: "Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection." in: Innate immunity, Vol. 22, Issue 2, pp. 103-12, 2016 (Sample species: Human). Further details: In vitro Assay,Blocking Peptide,Neutralization

Kopp, Kupsch, Schromm: "Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling." in: Biochimica et biophysica acta, Vol. 1863, Issue 4, pp. 660-72, 2016 (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence

Schmid, Novacek, Vogelsang, Papay, Primas, Eser, Panzer: "Platelets Toll-like receptor-4 in Crohns disease." in: European journal of clinical investigation, 2016

Imai, Fujita, Kajiya, Ouhara, Yoshimoto, Matsuda, Takeda, Kurihara: "Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 5, pp. 1777-1786, 2016

Moreira, Costa-Pereira, Alves, Marteleto, Ribeiro, Peruhype-Magalhães, Giunchetti, Martins-Filho, Araújo: "Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes." in: Veterinary parasitology, Vol. 220, pp. 33-45, 2016 Method employed by authors: Flow Cytometry (FACS)

Song, Fullerton, Ao, Zheng, Zhao, Meng: "BMP-2 and TGF-β1 mediate biglycan-induced pro-osteogenic reprogramming in aortic valve interstitial cells." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 4, pp. 403-12, 2015 (Sample species: Human). Further details: Functional Studies,Blocking Peptide,Neutralization

Jamin, Dehoux, Dossier, Fila, Heming, Monteiro, Deschênes: "Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome." in: Clinical and experimental immunology, Vol. 182, Issue 3, pp. 332-45, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Youn, Shim, Lee, Ji, Oh, Hong, Lee, Park, Lee, Choi, Chung, Kang: "Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis." in: Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, Vol. 23, Issue 3, pp. 139-44, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Feng, Feng, Xing, Shen, Tan, Huang, Lu, Tao, Zhang, Li, Gu: "Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs)." in: Cell and tissue research, Vol. 356, Issue 2, pp. 369-80, 2014 (Sample species: Human). Further details: In vitro Assay,Blocking Peptide,Neutralization

Komine-Aizawa, Hirohata, Aizawa, Abiko, Hayakawa: "Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine." in: Placenta, Vol. 36, Issue 1, pp. 27-33, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Rojas-Bernabé, Garcia-Hernández, Maldonado-Bernal, Delegado-Dominguez, Ortega, Gutiérrez-Kobeh, Becker, Aguirre-Garcia: "Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4." in: Parasitology, Vol. 141, Issue 6, pp. 788-800, 2014 (Sample species: Human).

Käbisch, Mejías-Luque, Gerhard, Prinz: "Involvement of Toll-like receptors on Helicobacter pylori-induced immunity." in: PLoS ONE, Vol. 9, Issue 8, pp. e104804, 2014 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Yuan, Zhou, Wang, Li, Xie, Zhao, Xu, Shen: "Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production." in: Cell death & disease, Vol. 4, pp. e794, 2013 (Sample species: Human).

Harman, Bye, Nasr, Sandgren, Kim, Mercier, Botting, Lewin, Cunningham, Cameron: "Identification of lineage relationships and novel markers of blood and skin human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 1, pp. 66-79, 2012

Wu, Chi, Hsieh, Luo, Yang: "TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2." in: Journal of cellular physiology, Vol. 223, Issue 2, pp. 480-91, 2010

Pillay, Ramakers, Kamp, Loi, Lam, Hietbrink, Leenen, Tool, Pickkers, Koenderman: "Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia." in: Journal of leukocyte biology, Vol. 88, Issue 1, pp. 211-20, 2010

Matsunaga, Tsuchimori, Matsumoto, Ii: "TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules." in: Molecular pharmacology, Vol. 79, Issue 1, pp. 34-41, 2010

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN4360113) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using the PE conjugate of 7. Stain...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using Azide/BSA FREE version of 7....
Immunoprecipitation (IP) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Immunoprecipitation: TLR4 Antibody (HTA125) - Analysis using the Alexa Fluor (R) 647...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using the Alexa Fluor (R) 647 conj...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using Azide/BSA FREE version of 7....
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using Azide/BSA FREE version of 7....
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Flow Cytometry: TLR4 Antibody (HTA125) - Analysis using Azide/BSA FREE version of 7....
 image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN4360113) Blocking/Neutralizing: TLR4 Antibody (HTA125) - Analysis using Azide Free version of...