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|Antigen||Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CAMK2A antibody is un-conjugated Alternatives|
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
Product Details anti-CAMK2A AntibodyTarget Details CAMK2A Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN2815316|
|Specificity||This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).|
|Predicted Reactivity||Human, Mouse, Rat|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.|
|Plasmids, Primers & others|
Target Details CAMK2AProduct Details anti-CAMK2A Antibody Application Details Handling Images back to top
|Alternative Name||CAMK2A (CAMK2A Antibody Abstract)|
|Background||CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain|
|NCBI Accession||NP_057065, NP_741960|
|Pathways||WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction|
Application DetailsProduct Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
DS WB Results: Approx 58-60 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3 kDa according to Human NP_057065.2, and 54.1 kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50 kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3 μg/mL. Primary incubation 1 hour at room temperature.
DS IHC Results: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.
Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.
Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
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