anti-Mouse (Murine) CAMK2A antibody for Immunofluorescence

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin dependent protein kinase II alpha
  • calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2A
  • camk2a.S
  • camk2a
  • Camk2a
Epitope
pThr286
27
26
12
10
10
9
7
6
6
5
5
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
143
94
92
26
10
6
5
4
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
115
58
19
Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
7
7
7
6
6
6
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
188
97
73
56
45
36
27
10
7
6
4
2
2
Supplier
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Catalog No. ABIN197054
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN214329 ELISA IF IHC IHC (p) WB Rabbit AA 6-23 Log in to see Polyclonal 0
1 ABIN197550 IF WB Rabbit Thr286 Log in to see Polyclonal 1
1 ABIN498462 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN498753 IF IHC (p) WB Rabbit pThr286 Log in to see Polyclonal 0
1 ABIN1732581 IF IHC (p) ELISA WB Rabbit IgG AA 6-23 Log in to see Polyclonal 0
1 ABIN466895 ELISA IF IHC IHC (p) IP RIA WB Mouse IgG1 Log in to see 6G9 0
1 ABIN1100390 IF WB Mouse IgG1 AA 281-294 Log in to see 22B1 0
1 ABIN212242 IF IP IHC RIA ELISA WB Mouse IgG1 Log in to see 0
1 ABIN5550934 EIA IF IHC (fro) IP RIA WB Mouse IgG1 Log in to see 6G9 0
1 ABIN1968172 ELISA IF IHC IHC (p) IP RIA WB Atto 594 Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968230 ELISA IF IHC IHC (p) IP RIA WB Biotin Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968313 ELISA IF IHC IHC (p) IP RIA WB Atto 594,PE Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968331 ELISA IF IHC IHC (p) IP RIA WB PE Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968322 ELISA IF IHC IHC (p) IP RIA WB PerCP Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968145 ELISA IF IHC IHC (p) IP RIA WB Atto 390 Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968163 ELISA IF IHC IHC (p) IP RIA WB Atto 565 Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968190 ELISA IF IHC IHC (p) IP RIA WB Atto 655 Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968208 ELISA IF IHC IHC (p) IP RIA WB Atto 700 Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968136 ELISA IF IHC IHC (p) IP RIA WB APC Mouse IgG1, kappa Log in to see 6G9 0
1 ABIN1968181 ELISA IF IHC IHC (p) IP RIA WB Atto 633 Mouse IgG1, kappa Log in to see 6G9 0

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope pThr286
(27), (26), (12), (10), (10), (9), (7), (6), (6), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(143), (94), (92), (26), (10), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(115), (58), (19)
Clonality
Polyclonal
Conjugate This CAMK2A antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(188), (97), (73), (56), (45), (36), (27), (10), (7), (6), (4), (2), (2)
Pubmed 3 references available
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling References for anti-CAMK2A antibody (ABIN197054) Images
Specificity This antibody detects endogenous levels of CaMKII only when phosphorylated at Threonine 286.
Purification Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).
Plasmids, Primers & others

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling References for anti-CAMK2A antibody (ABIN197054) Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968
Gene ID 815
NCBI Accession NP_741960
UniProt Q9UQM7
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling References for anti-CAMK2A antibody (ABIN197054) Images back to top
Application Notes Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details References for anti-CAMK2A antibody (ABIN197054) Images back to top
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-CAMK2A antibody (ABIN197054)

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling Images back to top
Background publications

Salomäki, Vinokurov, Kankare: "Effect of temperature on the buildup of polyelectrolyte multilayers." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 21, Issue 24, pp. 11232-40, 2005

Runyan, Moore, Dash: "A role for prefrontal calcium-sensitive protein phosphatase and kinase activities in working memory." in: Learning & memory (Cold Spring Harbor, N.Y.), Vol. 12, Issue 2, pp. 103-10, 2005

Pak, Huang, Li, Balschun, Reymann, Chiang, Westphal, Huang: "Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: a study with knockout mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11232-7, 2000

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling References for anti-CAMK2A antibody (ABIN197054) back to top
Supplier Images
 image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibody (ABIN197054) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibody
 image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibody (ABIN197054) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibody (Image 2)